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MAGUS Lum VD500L Fluorescence Inverted Digital Microscope

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With a camera. Magnification: 100–400x. Trinocular head, plan achromatic objectives, 5W LED transmitted light and reflected light illumination, Köhler illumination

Trinocular
The microscope head is a core element of the microscope that you look through to see a magnified sample
100 — 400
How much the size of a sample increases when you observe it through a microscope
Laboratory/medical
Application and use of the microscope
Availability

Product ID 83022
Brand MAGUS
Warranty 5 years
EAN 5905555018249
Package size (LxWxH) 38x61x82 cm
Shipping Weight 26.7 kg
Description

The MAGUS Lum V500L is an inverted microscope for fluorescence microscopy in the reflected light and brightfield and phase contrast microscopy in the transmitted light. DAPI, FITC, and TRITC fluorochrome filters are used for fluorescence microscopy. The inverted design allows you to study samples in laboratory ware such as petri dishes, flasks, etc. The height of the lab ware can be 55mm or 165mm when the stand is tilted. The optics are designed for lab ware with a bottom thickness of 1.2mm. The microscope is ideal for research and routine laboratory work and it can be used for educational purposes.

Digital Camera

The MAGUS CLM90 digital camera is designed for use in fluorescence and darkfield microscopy techniques.
The camera is equipped with a 7.1MP sensor and produces realistic images at a resolution of 3200x2200px. The camera is recommended for use with 4x, 10x, 20x, and 40x objectives. When working with low magnification objectives, the camera will allow you to see the finer details.
Video is recorded at 51.3fps or 133.8fps depending on its resolution. Videos are smooth with soft and subtle transitions between frames. The movement of the sample is displayed in real time with no delays. The camera makes it easy to work with objects that are moving and is suitable for classroom demonstrations.
The camera is equipped with a USB3.0 interface. The data transfer speed is 10 times faster than USB2.0 cameras. The high-speed camera is recommended for professional laboratories, research, or university training.

Optics

Two options for mounting a digital camera and monitor: The trinocular tube is for mounting a monitor (not included), and the side port on the body is for mounting a digital camera (not included). There is beam splitting: 100/0 or 0/100 (on the body) and 100/0 or 50/50 (trinocular head). The 45° eyepiece tube inclination angle allows for observation with maximum comfort. When assembling the microscope, the user can rotate the eyepiece tubes 180°.

The set includes six plan achromatic objectives: three of them for brightfield and fluorescence microscopy, and another three for phase-contrast microscopy. They are infinity-corrected and have a long working distance. The revolving nosepiece has six slots for mounting objectives. The revolving nosepiece is located under the stage on a stand.

Illumination

The fluorescence excitation source is a 5W LED with 50,000 hours of lifetime without replacement. There are three excitation filters: DAPI, FITC, and TRITC fluorochrome filters. LED illumination is more energy efficient and easier to maintain than mercury illumination, which is why it is recommended for use in universities. LED illumination provides color consistency. The LEDs turn on and off quickly and do not overheat during long hours of operation.

The source of the transmitted light is also a 5W LED. It is bright enough for brightfield and phase contrast microscopy techniques with each objective.

Both reflected and transmitted illumination can be adjusted according to the Köhler method. It allows you to reach the maximum resolution limit for each objective lens and get a clear, sharp image without artifacts or darkening at the edges.

The microscope is equipped with a phase-contrast condenser: Three slots are used for phase objectives with magnifications of 10x, 20x, and 40x, and the fourth slot is for brightfield microscopy. Phase rings can be centered. You can switch between the microscopy techniques by rotating a dial.

Stage and focusing mechanism

The fixed stage is equipped with a mechanical attachment for moving laboratory ware and three cup holders of different sizes. The lab ware can be moved in two mutually perpendicular directions in the horizontal plane. The microscope has a coarse and fine focusing mechanism. The coarse focusing mechanism has a lock knob and tension adjustment. The knobs are ergonomically designed to allow the user to maintain a relaxed posture while working.

Accessories

The microscope can be equipped with additional eyepieces, calibration slides, or a digital camera from the MAGUS line of accessories.

Key features of the microscope:

  • Inverted design for observing samples in lab ware with a bottom thickness of 1.2mm and samples of up to 55mm height in as well as up to 165mm height if the stand is tilted up
  • Microscopy techniques: fluorescence (reflected light), brightfield and phase-contrast (transmitted light)
  • Köhler illumination setup for transmitted and reflected light; long-lasting 5W LEDs without replacement as light sources
  • There are three excitation filters: DAPI, FITC, and TRITC fluorochrome filters
  • Phase-contrast condenser for quick and convenient switching between the microscopy techniques
  • Trinocular head; two options to mount a camera or monitor: the 100/0 or 0/100 beam splitting (on the body), 100/0 or 50/50 beam splitting (on the head)

Key features of the camera:

  • For fluorescence and darkfield microscopy with 4x, 10x, 20x, or 40x objectives
  • 7.1MP resolution: When observing with low magnification objectives, the camera will enable you to see finer details
  • 51.3fps and 133.8fps at 3200x2200px and 1584x1100px resolutions for observing moving objects, recording video and moving the preparation without jerkiness and delays
  • Peltier element reduces the sensor temperature during prolonged operation and eliminates thermal noise and, therefore, long shutter speeds can be used when taking photos
  • Global shutter for fast signal readout from the sensor, increased image brightness, no false optical effects when observing moving objects
  • SONY Exmor monochrome CMOS backlit sensor provides low noise level and high light sensitivity even in low-light conditions. You will get clearer, brighter, and more color-saturated images
  • USB3.0 interface for fast, smooth data transfer
  • Software with photo, video recording, editing, external display functions, linear and angular measurements

The kit includes:

  • MAGUS CLM90 Digital Camera (digital camera, USB cable, 12V, 3A adapter, case, Installation CD with drivers and software, user manual and warranty card)
  • Base with a built-in power input, reflected light illuminator, transmitted light source, condenser, focusing mechanism, stage, and revolving nosepiece
  • Trinocular head
  • Infinity plan achromatic objective: PLL 10x/0.25 phase WD 4.3 mm
  • Infinity plan achromatic objective: PLL 20х/0.40 WD 8.0mm
  • Infinity plan achromatic objective: PLL 40х/0.60 WD: 3.5mm
  • Infinity plan achromatic objective: PLL 10x/0.25 PHP2 WD 4.3mm
  • Infinity plan achromatic objective: PLL 20x/0.40 PHP2 WD 8.0mm
  • Infinity plan achromatic objective: PLL 40x/0,60 PHP2 WD 3.5mm
  • Eyepiece 10x/22mm with long eye relief (2 pcs.)
  • UV shield
  • C-mount adapter 1x
  • Hex key wrench
  • Power cord
  • Dust cover
  • User manual and warranty card

Available on request:

  • 10x/22mm eyepiece with a scale
  • 12.5x/14mm eyepiece (2 pcs.)
  • 15x/15mm eyepiece (2 pcs.)
  • 20x/12mm eyepiece (2 pcs.)
  • 25x/9mm eyepiece (2 pcs.)
  • Calibration slide
Specifications
Product ID 83022
Brand MAGUS
Warranty 5 years
EAN 5905555018249
Package size (LxWxH) 38x61x82 cm
Shipping Weight 26.7 kg
Type biological, light/optical
Head trinocular
Nozzle Siedentopf, rotatable 180°
Magnification, x 100 — 400
Magnification, x (optional) 40–500/600/800/1000
Eyepiece tube diameter, mm 30
Eyepieces 10х/22mm, eye relief: 10mm (*optional: 10x/22mm with scale, 12.5x/14; 15x/15; 20x/12; 25x/9)
Objectives infinity plan achromatic: PLL 10x/0.25/4.3; PLL 20x/0.40/8.0; PLL 40x/0.60/3.5; phase: PLL 10x/0,25/4,3 PHP2; PLL 20x/0.40/8.0 PHP2; PLL 40x/0.60/3.5 PHP2; parfocal distance: 45mm
Revolving nosepiece for 6 objectives
Working distance, mm 4.3 (10х); 8.0 (20x); 3.5 (40x)
Interpupillary distance, mm 48 — 75
Stage, mm 227x208
Stage moving range, mm 77/134.5
Stage features fixed, with glass plate Ø118mm and mechanical attachment; dish holders: 86x129.5, Ø90mm; 34x77.5mm, Ø68.5mm; 57x82mm, Ø60mm; 29x77.5mm, Ø35mm
Condenser NA 0.6, working distance: 55mm; phase contrast turret; with locking screws
Diaphragm aperture iris diaphragm
Focus coaxial coarse and fine focusing knobs located on both sides
Illumination halogen, fluorescent
Brightness adjustment
Power supply AC network, 85–265V, 50/60Hz
Light source type reflected light: 5W LED; transmitted light: 5W LED
Light filters yes
Operating temperature range, °C 5...+35
Special features phase contrast condenser (turret) with a free slot and phase annuli plates for 10x, 20x and 40x objectives; centering auxiliary microscope
User level experienced users, professionals
Assembly and installation difficulty level complicated
Fluorescent module filters: ultraviolet (UV), violet (V), blue (B), green (G)
Fluorescence filter: filter type, excitation wavelength/dichroic mirror/emission wavelength ultraviolet (UV), 320–380nm/425 nm/435 nm; violet (V), 380–415nm/455nm/475nm; blue (B), 450–490nm/505nm/515nm; green (G), 495–555nm/585nm/595nm
Application laboratory/medical
Illumination location dual
Research method bright field, fluorescence, phase-contrast microscopy
Pouch/case/bag in set dust cover
Camera specifications
Sensor SONY Exmor CMOS
Color/monochrome monochrome
Megapixels 7.1
Maximum resolution, pix 3200x2200
Sensor size 1.1" (14.4x9.9mm)
Pixel size, μm 4.5x4.5
Two-stage thermoelectric module (Peltier element) to set the temperature 42°C below room temperature
Light sensitivity 3354mV with 1/30s
Signal/noise ratio 0.15mV at 1/30s
Exposure time 0.1ms–1h
Video recording
Frame rate, fps at resolution 51.3@3200x2200, 133.8@1584x1100
ADC digit capacity (bit) 8/12 (selectable)
Image format *.jpg, *.bmp, *.png, *.tif
Video format output: *.wmv, *.avi, *.h264 (Windows 8 and later), *h265 (Windows 10 and later)
Shutter type Global shutter
White balance manual, automatic
Exposure control manual, automatic
Software features image size, brightness, exposure time
Software MAGUS View
Output USB 3.0, 5Gb/s
System requirements Windows 8/10/11 (32bit and 64bit), Mac OS X, Linux, up to 2.8GHz Intel Core 2 or higher, minimum 2GB RAM, USB 3.0 port, CD-ROM, 17" or larger display
Mount type C-mount
Body CNC aluminum alloy
Camera power supply DC, 5V, from computer USB port; a 12V, 3A adapter for Peltier element
Camera operating temperature range, °С -10...+50
Operating humidity range, % 30 — 80
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Questions and Answers
Marc Panas (2024.10.09)
Hi,

I have a question concerning the manual stage: Does the offer include adaptors so that it can be used for microscope slides, flasks (up to 75 cm2), multi-well plates and petri-dishes?

One more question. The camera comes with a software, However I could not find any software handbook in the download section.

Thanks a lot in advance for your time and efforts.
Kind regards
Customer service:
Hi Marc,

thanks for your query.

The object stage is fixed: 227x208 mm; it has a mechanical device for moving the sample and a glass insert Ø118 mm; cup holders: 86x129.5 mm, Ø87.5 mm; 34x77.5 mm, Ø68.5 mm; 57x82 mm, Ø60 mm.

The software for MAGUS CLM90 Digital Camera (included in the microscope kit) is available in the download section:
https://eu.levenhuk.com/catalogue/microscopes/magus-lum-vd500l-fluorescence-inverted-digital-microscope/?oid=55964#product-downloads

Besides, the kit includes an installation CD with drivers and software for the camera.

Hope this helps!
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