MAGUS Lum D400 Fluorescence Digital Microscope

Fluorescence microscope MAGUS Lum 400 is designed for diagnostics and research by methods of fluorescence (reflected light) and brightfield (transmitted light) microscopy. Darkfield, phase contrast, and polarization techniques can be used with optional equipment. Areas of application of fluorescence microscope: DNA analysis, study of pathogens, sanitary and epidemiological surveillance, forensic examination, etc.

Digital camera

The MAGUS CLM10 digital camera is designed for use in fluorescence and darkfield microscopy techniques. The camera is equipped with a 2.3MP sensor and produces realistic images at a resolution of 1920х1200px. It is recommended for 40x, 60x, and 100x objectives. When observing with medium magnification objectives, the camera will show finer details. Video is recorded at 120fps at maximum resolution. Videos are smooth, with soft and subtle transitions between frames. The movement of the object is displayed in real time with no delays. The camera makes it easy to work with objects that are moving and is suitable for classroom demonstrations. The camera is equipped with a USB3.0 interface. The data transfer speed is 10 times faster than USB2.0 cameras. The high-speed camera is recommended for professional laboratories, research, or university training.

Optics

This is a trinocular microscope: The binocular head is 30° inclined and the digital camera (not included) is installed in the trinocular tube. As the eyepiece tubes are 360° rotatable, it is easy to adjust them to fit the height of the user.

Five objectives can be installed in the revolving eyepiece. Four infinity plan achromatic objectives are included. One of them, a fluo objective with a 40x magnification, is designed for fluorescence observations. 4x, 10x, and 100x objectives are used for the brightfield microscopy technique. An additional objective can be mounted in the free slot of the revolving nosepiece to achieve extra magnification within the magnification range of the microscope.

The revolving nosepiece with objectives is oriented toward the interior – the user can see the objective inserted into the optical path.

Illumination

The reflected light source is a 100W mercury lamp. It is placed inside the lamphouse, which provides efficient heat dissipation: The lamp does not overheat even during long hours of operation. The lamp can be centered along three axes to provide the optimal illumination of the working area. If you need to replace it, it will be fairly easy to remove and install the bulb. The mercury lamp is suitable for fluorescent dyes because it has discrete peaks. The reflected light illuminator contains four excitation filters: ultraviolet (UV), violet (V), blue (B), and green (G).

Transmitted light source: 30W halogen bulb. It is bright enough to work with any objectives of any magnification. Thanks to the color temperature of the illumination, even long observations will not strain the eyes. The illumination system also includes a condenser. It has a slot for a slider (phase contrast or darkfield).

In transmitted and reflected light, you can set up the Köhler illumination method. When the illumination is set up in this way, there is no darkening at the edges, the sample is clearly visible, and the image artifacts are removed.

Stage and focusing mechanism

The microscope is equipped with an ergonomically designed stage without a positioning rack. When observing in reflected light, a black stage plate is placed on the stage to remove stray light. The specimen holder is removable and can be taken out, if necessary, by loosening two screws. The focusing knobs are coaxial and located on each side of the microscope. Coarse focusing has a lock knob and tension adjustment and fine focusing is used for focus adjustment.

Accessories

The MAGUS Lum 400 microscope can be equipped with additional accessories: digital camera, phase contrast device, polarization device, darkfield condenser, eyepieces and objectives, darkfield and phase contrast sliders. For measuring objects, the configuration can be supplemented with a calibration slide.

Key features of the microscope:

• Microscopy techniques: fluorescence in reflected light, brightfield in transmitted light
• Other research methods with optional accessories: phase contrast, simple polarization, darkfield
• Trinocular head with a trinocular tube for mounting a digital camera; 360° rotatable eyepiece tubes
• Infinity plan achromatic objectives, including a fluo objective
• Reflected light source: 100W mercury lamp, transmitted light source: 30W halogen lamp
• Köhler illumination method in transmitted and reflected light
• Fluorescence filters: ultraviolet (UV), violet (V), blue (B), green (G)
• Ergonomic stage with a removable specimen holder and no positioning rack

Key features of the camera:

• For fluorescence and darkfield microscopy with 40x, 60x, and 100x objectives
• 2.3MP resolution for getting detailed images at high magnification; low noise level, low-power dissipation
• 120fps for observing moving objects, recording video, and moving the sample without jerkiness or delays
• Global shutter for fast signal readout from the sensor, increased image brightness, and no false optical effects when observing moving objects
• SONY Exmor monochrome CMOS backlit sensor provides low noise level and high light sensitivity even in low-light conditions. You will get clearer, brighter, and more color-saturated images.
• USB3.0 interface for fast, smooth data transfer
• Software with photo, video recording, editing, external display functions, linear and angular measurements

The kit includes:

• MAGUS CLM10 Digital Camera (digital camera, USB cable, installation CD with drivers and software, user manual and warranty card)
• Stand with transmitted light source, focusing mechanism, stage, condenser mount, and revolving nosepiece
• Abbe condenser
• Reflected light illuminator
• Mercury lamphouse
• Trinocular head
• Infinity plan achromatic objective: PL 4x/0.10 WD 19.8mm
• Infinity plan achromatic objective: PL 10x/0.25 WD 5.0mm
• Infinity plan achromatic objective, fluo: PL FL 40x/0.85 (spring-loaded) WD 0.42mm
• Infinity plan achromatic objective: PL 100x/1.25 (spring-loaded, oil) WD 0.36mm
• Eyepiece 10x/22mm with long eye relief (2 pcs.)
• Eyepiece eyecup (2 pcs.)
• UV shield
• Filter slider
• Black protective plate for under-stage installation
• C-mount camera adapter
• Color filter (4 pcs.)
• Bottle of immersion oil
• Mercury lamp power supply
• AC power cord (2 pcs.)Mercury lamphouse power cord
• Dust cover
• User manual and warranty card

Available on request:

• 10x/22mm eyepiece with a scale
• 12.5x/14mm eyepiece (2 pcs.)
• 15x/15mm eyepiece (2 pcs.)
• 20x/12mm eyepiece (2 pcs.)
• 25x/9mm eyepiece (2 pcs.)
• Infinity plan achromatic objective, fluo: PL FL 10x/0.35 WD 2.37mm
• Infinity plan achromatic objective: PL 20х/0.40 WD 8.8mm
• Infinity plan achromatic objective: PL 60x/0.80 ∞/0.17 WD 0.46mm
• Phase contrast device
• Darkfield condenser NA 0.9
• Oil darkfield condenser NA 1.36–1.25
• Darkfield slider
• Polarization device
• Calibration slide
• LCD Monitor