MAGUS Bio VD360 Biological Inverted Digital Microscope

The research-grade microscope. The microscope is designed for studying liquid precipitates, cell colonies, living cells, tissue cultures, and other stained and unstained specimens in laboratory glassware. The inverted design of the microscope involves the use of Petri dishes, multiwell plates, bottles, roller bottles, and flasks up to 75mm with a bottom thickness of 1.2mm. The microscope uses special objectives to work with such glassware. With the condenser removed, you can observe cell cultures in Petri dishes or in cylindrical flasks up to 187mm tall. Observations are made in transmitted light using the brightfield, phase contrast, and emboss contrast techniques. Optional components will allow for the use of the Hoffman modulation contrast techniques. The microscope is equipped with a 5MP camera with a USB 3.0 interface. The camera connects to a computer and displays the image on its screen. The software complements the system with analysis and documentation functions. The camera mounts to the microscope using either the 0.5x or 1x adapter that is included with the microscope. The adapter is selected based on the tasks to be solved. The 0.5x adapter extends the field of view, while the 1x adapter transfers the image “as is”, while cropping the edges, but each detail appears larger on the sensor. The microscope is used for research in medicine, pharmacology, biology, and virology.

Digital camera

MAGUS CBF90 is a science camera with 5MP resolution and a 2/3" sensor. The camera produces a realistic image in 2448x2048px. Video is recorded at 35fps or 50fps depending on its resolution. Videos are smooth, with soft and subtle transitions between frames. The movement of the sample is displayed in real time with no delays. The camera provides convenient work with moving objects and is suitable for conducting demonstrations in a classroom. The camera is equipped with a USB 3.0 interface. The data transfer rate is 10 times faster than that of USB 2.0 cameras. The high-speed camera is recommended for professional laboratories, research, or university training.

Microscope head

Binocular, with infinity-corrected optics. The digital camera is mounted in the side camera port on the microscope head. The light splitting ratio is 100/0 or 0/100. A slot with a plug is intended for installing the emboss contrast slider.

Revolving nosepiece

Coded, for 5 objectives. An additional objective can also be installed in the free slot in order to achieve extra magnification.

Objectives

Infinity-corrected plan achromatic objectives with an extended working distance have been corrected for a dish bottom thickness of 1.2mm. The parfocal distance is 60mm. The 4x objectives are used for the brightfield technique. The 10х, 20х, and 40х objectives are phase objectives that differ from the 4x objectives by having a phase ring in the plane of the exit pupil. These objectives are designed for phase contrast observations, but they are also suitable for the brightfield and emboss contrast techniques.

Maintaining comfortable brightness levels when switching magnifications

The objectives of different magnifications transmit light with different levels of intensity, and so each time you change objectives, the brightness of the light must be adjusted. Switching from a higher to a lower magnification objective causes eye fatigue, as the image brightness in the eyepieces increases sharply. The MAGUS Bio VD360, which is equipped with intelligent brightness control, solves this problem. The microscope remembers the brightness for each objective that the user has selected and automatically sets this brightness when turning the nosepiece. Intelligent control reduces the time required to adjust brightness. MAGUS Bio VD360 increases user comfort and saves time even when work requires frequent magnification changes.

Focusing mechanism

The coarse and fine focusing knobs are coaxial and located low. The researcher can place their hands on the table and take a comfortable position in front of the microscope. The ring on the right side adjusts the tension of the coarse focusing travel. The user adjusts the comfortable tension for work.

Stage

The stage is fixed. A special mechanism is installed on the stage, which moves laboratory glassware in two mutually perpendicular directions. The smooth and subtle movement of the object augments the accuracy of the study: no part of the specimen will be overlooked. The long stage control handle ensures user comfort: The hand rests on the table with no strain. The microscope kit includes a universal labware holder.

Condenser

The condenser has 0.3 NA and a working distance of 75mm. A phase contrast slider or an emboss contrast slider with a sector diaphragm is installed in the condenser slot. A phase contrast slider is a plate with three holes. One hole has a phase insert for 10x, 20x, and 40x objectives, while the other two are used for brightfield observations. The annular diaphragm of the slider is aligned with the phase ring of the objective using a centering telescope. An emboss contrast slider with a sector diaphragm is also a plate with three holes. The emboss contrast microscopy is enabled by introducing the sector diaphragm into the optical path and setting the slider in the microscope head slot to the position that matches the objective being used. The green filter (ND6), when placed into the slot with the sector diaphragm, removes color halos at the edges of the observed structures. The slider’s free holes are designed for brightfield observations: one for a 4x objective and the second one for 10х, 20х, and 40х objectives.

Light source

The 3W LED delivers bright specimen illumination that is sufficient for all of the microscope’s available techniques and objectives. The color temperature does not change when you adjust the brightness. The LED lifetime is 50,000 hours.

Status LCD screen

The LCD screen on the base displays the objective magnification, light source brightness, and an operation mode (Sleep, Eco). The screen and a single knob allow the user adjust the brightness, lock the brightness adjustment, set the Sleep mode and the auto-off timer.

Ergonomic design

Physical discomfort causes fatigue and reduces productivity. The ergonomic design of the microscope plays an important role in everyday scientific research. MAGUS Bio VD360 provides user comfort during work. The microscope head is located at an ergonomic angle so that your back and neck do not get tired. Thanks to the low and compact stage, it is convenient for the user to manipulate the sample and work with laboratory glassware. The long handle of the movement mechanism makes it so that you do not need to raise your hand from the table to control the microscope, nor to change your comfortable position. Focusing knobs are located at the bottom of the body. The user does not need to strain their hands. Thanks to the smooth movement of the mechanism, the user can effortlessly focus on the object.

Accessories

There is a line of accessories designed specifically for this microscope. Additional objectives without phase rings ensure optimal image quality when working in brightfield. Eyepieces extend the magnification range of the microscope. Additional eyepieces will help use the full potential of an objective that is used more often. The phase contrast device with plan semi-apochromatic objectives is suitable for fundamental and applied research. Plan semi-apochromatic objectives provide a high degree of aberration correction, producing clear, bright, and high-contrast images. Hoffman modulation contrast enables you to visualize the fine details in live specimens with high contrast and without halos, allowing the study of dynamic processes in real time. A calibration slide is used to measure objects and can be combined with the eyepiece with a scale or with the camera software. Dish holders and stage inserts are used for the convenient installation of culture bottles of various types and sizes on the microscope.

Alternative models

The MAGUS Bio VD360 is an alternative for the following models: Carl Zeiss Primovert, Leica DM IL LED, Leica DMi1, Nikon ECLIPSE Ts2, Nikon ECLIPSE Ts2-F, Nikon ECLIPSE Ts2R, Olympus CKX53, Olympus IX53.

Microscope features:

• Research of stained and unstained objects in laboratory glassware: Petri dishes, flasks, plates, work with glassware up to 187mm in height
• Microscopy techniques: brightfield, phase contrast, and emboss contrast; with optional accessories: Hoffman modulation contrast.
• Coded revolving nosepiece: The brightness of the light source is set automatically depending on the selected objective
• Binocular head with side tube for mounting a digital camera; 100/0 or 0/100 beam splitting
• Transmitted light illuminator: energy-saving 3W LED with a lifetime of up to 50.000 hours
• Condenser with a slot for installing sliders; phase-contrast slider included, phase rings are centered
• Smart lighting control system: automatic brightness selection, dimming lock, timer auto-off, LCD operating status screen
• Stage with glassware movement along the X and Y axes, a universal dish holder included, a long stage control handle ensures user comfort while working

Camera features:

• Science camera for brightfield observations with 20x, 40x, 60x and 100x objectives
• Large sensor size – 2/3'': better image quality, less noise level, more light sensitivity
• 5MP resolution: when observing with medium magnification objectives, the camera will show more fine details
• 35fps or 50fps depending on the resolution for observing moving samples, recording video, and moving the preparation without jerks or lags
• SONY Exmor backlit color CMOS sensor delivers low noise performance and high light sensitivity even in low light conditions: clearer, brighter, and more color-saturated images
• USB 3.0 interface for fast data transfer with no jerking or lagging
• Software with photo, video recording, editing, external display, linear and angular measurements

Package:

• MAGUS CBF90 digital camera (digital camera, USB cable, USB flash drive with drivers and software, user manual, and warranty card)
• Stand with a built-in power supply, transmitted light source, focusing mechanism, stage, condenser mount, and revolving nosepiece
• Condenser with slider slot
• Binocular microscope head with side camera port
• Plan achromatic objective Plan 4x/0.10 ∞/1.2 WD 30mm, parfocal distance: 60mm
• Plan achromatic objective Plan 10х/0.25 phase ∞/1.2 WD 10.2mm, parfocal distance: 60mm
• Plan achromatic objective Plan 20х/0.40 phase ∞/1.2 WD 12mm, parfocal distance: 60mm
• Plan achromatic objective Plan 40х/0.60 phase ∞/1.2 WD 2.2mm, parfocal distance: 60mm
• 10х/22mm eyepiece with long eye relief and diopter adjustment (2 pcs.)
• Eyecup (2 pcs.)
• Centering telescope
• Phase-contrast slider with centerable phase annuli
• Emboss contrast device
• Green filter
• Universal labware holder
• 0.5x C-mount adapter
• 1х C-mount adapter
• Microscope power cord
• Dust cover
• User manual and warranty card

Available on request:

• Plan achromatic objective Plan 10х/0.25 ∞/1.2 WD 10.2mm, parfocal distance: 60mm
• Plan achromatic objective Plan 20х/0.40 ∞/1.2 WD 4mm, parfocal distance: 60mm
• Plan achromatic objective Plan 40х/0.60 ∞/1.2 WD 2.2mm, parfocal distance: 60mm
• 10х/22mm eyepiece with a scale
• 10x/22mm eyepiece with a center field pointer
• 10x/22mm eyepiece with a reticle
• 12.5х/16mm eyepiece (2 pcs.)
• 15х/16mm eyepiece (2 pcs.)
• 20х/12mm eyepiece (2 pcs.)
• 30х/8mm eyepiece (2 pcs.)
• Phase contrast device: phase-contrast sliders, set of plan semi-apochromatic phase objectives
• Hoffman modulation contrast device with objective set
• Glass stage plate
• Metal stage plate for working with a culture bottle
• Labware holder kit
• Calibration slide
• Monitor and digital camera with an HDMI interface
• Set of color filters (blue, green, yellow, frosted glass)